The major advantage of SMRT sequencing over other technologies is the long read-length. Other major benefits of the platform, such as improved de novo assembly and detection of structural variation are derived from this advantage. With more and more evidence pointing to the importance of structural variation between different genomes, PacBio’s long reads put it in a unique market position.
While a lot of useful information can be gleaned from analyzing DNA, it can represent a somewhat static view of the organism. Outside of somatic mutations, DNA sequences change very little throughout the organism’s life cycle. Life is very dynamic by nature - being able to explore the constant change happening as an organism responds to its environment is a crucial part of the whole picture.
Metagenomics is a very broad field that can often be summarized down to the analysis of a microbiome. These microbiomes are all around and inside us. They’re in the air we breathe, the water we drink and the food we eat.
The advent of next-generation sequencing has revolutionized biology and many other fields. Sequencing is now used to develop new drugs, understand personal ancestry and predisposition to certain diseases, improve yields in agriculture, find new oil fields, and in many other areas. DNA sequencing is becoming as indispensable as computing in many industries.
When you are looking for a sequencing facility there are two main factors to consider aside from the quality of the data cost and turn-around time (TAT). We've researched many facilities across the globe and this is what we have found:
First, the cost of sequencing varies widely between facilities. In part, this is due to the facility's source of funding: governmental or private. Most government funded academic institutions run their sequencers at cost for their internal users (students and professors). They usually have a pricing structure as follows: cheapest for internal, more expensive for external nonprofit users and the most expensive (sometimes as much as a 50% surcharge) for external for-profit users. To illu
Pacific Bioscience builds a platform that can sequence long reads on the range of 1000 - 60000 bp significantly faster than short read sequencing. It uses a SMRT cell (Single Molecule Real-Time) sequencing technology - a Zero Mode Waveguide (ZMW) well that is smaller than the light wavelength that captures the emission of a fluorophore being cleaved off a dNTP while a polymerase attaches it to a DNA sequence. The real time dynamics allow monitoring of the time between two base incorporations informing about base modifications like m6A and m4C. The
In the fall of 2016, NGX Bio announced the winners of NGS discovery funding contest at Festival of Genomics event in San Diego.The abstracts were judged by a team of NGX Bioscientists on scientific merit, innovation, and use of NGS in their research.
The winner of the grand prize is Christina Bergey and Kenny Chiou from Washington University. Their proposal utilized a non-invasive method to extract high-quality genome from a low-quality fecal sample from an endangered western lowland gorilla. They will use the 5k prize to test their cutting edge technology for non-invasive genome sequencing from fecal Gorilla samples. Non-invasive genomics is key for environmental monitoring as well as for conservation and forensic application.
The first runner-up winner is González-Segovia Eric Gerardo from
The goal of the modern genomics researcher is not simply to build genomics tools or even to gather genomic data. Instead, the ultimate objective is to further scientific knowledge to better understand human disease, plant and animal genetics, microbial ecology or evolution. The genomics researcher or bioinformatician seeks a deeper understanding of the science; better research tools are only a means to this end. A number of complex steps are involved in genomic sequencing, including platform or vendor selection, sample preparation, sequencing, data management and bioinformatics. Each of these individual processes bears its own learning curve, and a failure at any step could cripple downstream results. Next Generati