HLA Typing
Human leukocyte antigen (HLA) genes are the most polymorphic in the human genome). They play a pivotal role in the immune response and have been implicated in numerous human pathologies, especially autoimmunity and infectious diseases. When a mutation occurs in any of the 11 HLA loci, our body loses the ability to distinguish between self-cells and nonself-cells. Furthermore, mutations can cause transplant rejection, autoimmune responses, promotion of cancer, and drug sensitivity.
Genes/regions covered: Loci Target Sequence| HLA-A 4.1 kb (entire gene) | HLA-DQB1 7.1 kb (exon 1-3’UTR) |
| HLA-B 2.6 kb (exons 1-7 + introns) | HLA-DPB1 9.7 kb (exon 2-3’UTR) |
| HLA-C 4.2 kb (entire gene) | HLA-DQA1 7.3 kb (entire gene) |
| HLA-DRB1/3/4/5 4.1 kb (exon 2, intron 4) | HLA-DPA1 10.3 kb (entire gene) |
Applications
Identifying matches for transplants
High resolution