Solutions

We can sequence any organism.
Let us do the legwork.

01

Design

NGX Bio makes planning and executing your project easy. Our experts support you throughout the project -- from sample preparation to data delivery. We can also offer guidance, if it’s desired, on which protocols will be best for your project.

Online project management tools automatically generate quotes with defined turnaround times and status updates. We arrange for a courier to pick up your samples, which are tracked using barcoded tubes and plates.

02

Sample Preparation

We handle DNA and RNA extraction, library construction, library pooling, and a complete set of state-of-the-art quality control tests.

DNA and RNA Extraction: Depending on your needs, we can extract DNA or RNA, or just quantify and dilute your samples to appropriate concentration.

Library Construction: We make both single-indexed and dual-indexed sequencing libraries. Supported kits include TruSeq PCR Free, TruSeq Nano, Nextera, and a variety of exome capture kits. We also offer 10X genomics and Dovetail Genomics library preparation.

Quality Control: We use Qubit, PicoGreen, Bioanalyzer traces, or qPCR to determine library quality. Only high quality libraries are used for sequencing.

03

Sequencing

We can get your samples on the full range of sequencing platforms, from Illumina (HiSeq X Ten, HiSeq 2000, HiSeq 2500, HiSeq 4000, NextSeq 500, MiSeq) to PacBio (Sequel, RS II) and BioNano as well as 10X genomics Chromium machines.

No matter what read type (i.e. single end or paired end) or read length (from 50bp to 300bp) you need, we can quote a variety of options and identify the best fit for you. When your project is done we will provide you with automatic QC so you know that you can trust the data.

The sequence data are processed and analyzed using FastQC so that you know which samples to trust going forward.

A sequencing project is a significant logistical undertaking. Let us do the legwork.

04

Data Analysis

Your raw data will be hosted in secure and private silos in Amazon S3 cloud storage, where you can readily access it using rapid file transfer tools from Globus.

Command line access via Amazon CLI is available for scripting access in place. We can provide additional data encryption depending on your requirements. We provide economical long-term archiving of your files such that you can easily reanalyze your sequence data even years later.

We provide robust quality control to remove common mapping artefacts and pitfalls. We perform data analysis using pipelines that follow GATK best practices implemented in high performance scalable cloud clusters. From basic variant-calling to more sophisticated population genetic analyses, we support your research with the level of analysis you need.

Standard Sequencing

Whole Human Genome

  • - 30 X coverage, 2X150, HiSeq X
  • - TruSeq DNA PCR-free, TruSeq Nano
  • - Over 800 million reads, >80% mappable
  • - Quality control, Qubit, bioanalyzer, qPCR
  • - Bioinformatics : fastq, BAM, VCF, GATK

Human Exome

  • - 50X Coverage, 2x100 reads, HiSeq 2500
  • - 50 mbp, 488717 loci, Agilent SureSelect V5.5
  • - Over 30 million reads, 98% mappable
  • - Quality control, Qubit, bioanalyzer, qPCR
  • - Bioinformatics: fastq, BAM, VCF, GATK

Constructed
Libraries

  • - Multiple coverage ranges, all Illumina, Pac Bio machines
  • - Multiplex samples to save costs
  • - DNA, RNA Metagenomic analysis
  • - Quality control: Qubit, Bioanalyzer, qPCR
  • - Bioinformatics: fastq, vcf, GATK

Custom Sequencing

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